Use chem draw (or equivalent) to determine what pH the molecule is unionised (or least ionised) then use the appropriate buffered mobile phase to ensure the molecule is running on HPLC at that pH. May take into account the organic modifier effects on pH, depending on how close the ionisation points are for each group.
Ask advice and consider all options before making an informed decision
I would use different strategies to identify the bottleneck in protein expression, from mRNA to protein, checking the secretory pathway, also it has been shown that different host cells or clones present different ability to produce proteins
My answer was that such information is confidential and that I don't see why he needs to know my past salary to evaluate my talent and value. He kept insisting and told me that he is the one that makes the questions, then I told him that I was not interested in proceeding with the process.